电针对大鼠侧脑室注入红藻氨酸所致海马痫样丛状放电的影响

Effects of electroacupuncture on hippocampal epileptic burst discharges induced by intracerebroventricular injection of kainic acid in the rat

大鼠侧脑室注入红藻氨酸(Kainic Acid,KA)所致癫痫,可被电针“督脉穴”缓解。表现为:海马痫波幅度和频率均减低,第1min内主要以痫波幅度的显著降低为主。电针前后海马脑电未出现去同步化。侧脑室注入KA后,海马痫样放电分为正性单位、负性单位和无关单位三种形式,电针后正性单位被抑制,负性单位被激活,且正性单位(21％)、无关单位(33％)转为负性单位。同时电针使海马癫痫神经元丛状放电的每一丛状放电中峰电位数目(简称NSEB值)及丛内峰电位频率(简称IBSF值)减小。提示:(1)电针制痫可能是通过抑制海马癫痫脑电的振幅起作用;(2)海马负性单位是电针制痫的因素之一;(3)电针能调整癫痫神经元的固有特性。

Epilepsy induced by intracerebroventricular injection of kainic acid in the rat could be attenuated by electroacupuncture(EA)at the 'Du Mo point' since EA resulted in a reduction of the frequency and amplitude of the hippocampal EEG especially in the 1st min after EA.No unsynchronization of the hippocampal EEG occurred before or after EA.The hippocampal unit discharges after intracerebroventricular injection of kainic acid were classified into positive units,negative units and indifferent units,The positive units were inhibited by EA while the negative units,on the contrary,were activated.The positive units(21%)and the indifferent units(33%)were converted into negative units by EA.At the same time,EA reduced the NSEB value and IBSF value of hippocampal epileptic burst discharges.On the basis of these findings,it is believed that the inhibition of the amplitude of hippocampal epileptic EEG by EA plays an important role of the antiepileptic action of EA,hippocampal negative units are one of the factors of antiepileptic action,and EA can regulate the intrinsic characteristics of epileptic neurones.