重组人TNF对正常家兔致休克作用的研究——脂质过氧化作用和白细胞吞噬功能的改变

Study on the shock-inducing effect of recombinant human tumor necrosis factor:Changes of lipid peroxidation and leuco-cytic phagocytosis

给正常家兔静脉一次输注重组人肿瘤坏死因子(TNF)60μg/kg体重或130μg/kg体重后,血浆丙二醛(MDA)明显升高(P<0.01),血浆或溶血液超氧化物歧化酶(SOD)明显下降(P<0.05～0.01),上述变化在130μg/kg体重组要甚于60μg/kg体重组(P<0.05～0.01)。同时130μg/kg体重组动物其外周血白细胞数较对照组有明显下降(P<0.05～0.01),血浆调理时间明显缩短(P<0.01),此外白细胞的基础吞噬活性、基础总吞噬活性、最大吞噬活性和最大总吞噬活性均有明显改变(P<0.05～0.01)。上述结果表明重组人TNF能够引起正常家免的脂质过氧化损伤效应,以及白细胞数量和吞噬功能的改变。

After intravenous infusion of recombinant human tumor necrosis factor(TNF)in the dosage of 60μg/kg or 130μg/kg to rabbits,it was found that their plasma malondialde-hyde(MDA)was significantly increased(P<0.01),and superoxide dismutase(SOD)in plasma or hemolytic blood decreased(P<0.05~0.01)The increase of MDA and the decrease of SOD were more prominent in the 130μg group than in the 60μg group(P<0.05-0.01).In addition,in the 130μg group,the peripheral leucocytic count was decreased(P<0.05~0.01 as compared with the control)and the plasma opsonin time shortened(P<0.01).Moreover,the basic phagocytic activity,basic total phagocytic activity,maximun phagocytic activity and max-imun total phagocytic activity of leucocytes were all changed(P<0.05-0.01).These findings indicate that recombinant human TNF can induce tissue damages due to lipid peroxidation and changes of leucocytic count and phagocytic activities in normal rabbits.