人恶性肿瘤相关核仁抗原P_(24)在肿瘤病理诊断中的意义探讨

Study on the diagnostic significance of human malignancea-ssociated nucleolar antigen P_(24) for tumors

为初步探讨HMNA P_(24)在肿瘤病理学中的意义,本研究用抗P_(24) McAb MA_2以间接免疫荧光和ABC法对552例人体组织标本进行染色观察。结果表明,P_(24)可在97％的各种人恶性肿瘤细胞核仁中检出,而在98.6％的良性肿瘤组织核仁阴性;不典型增生组织有50％核仁染色阳性;正常人体组织和非肿瘤性病变组织细胞核仁全部阴性。说明P_(24)有较高的恶性肿瘤特异性,是区别良恶性肿瘤的较为有效的肿瘤标志物。

In order to assess the significance of human malignancy-associated nucleolar antigen P24 in the diagnosis of tumors,552 specimens of human tissues were stained and observed with immuno - fluorescent and ABC methods using anti-P24 monoclonal antibody MA2.It was found that P24 was positive in the nucleolus of 97% cases of malignant tumors and in 50% of atypical hyperplastic tissues but negative in 98.6% cases of benign tumors and in all the normal or nontumorous tissues.On the basis of these findings,it is believed that P24 is highly specific to malignant tumors and will be a useful marker for the differential diagnosis between malignant and benign tumors.