DNA-RNA双参数定量分析在早期诊断喉鳞癌中的意义

The significance of DNA-RNA double parameter quantitative analysis in the early diagnosis of laryngeal squamous cell carcinoma

本文采用计算机辅助图象分析,对33例喉鳞状上皮轻度增生、声带息肉、不典型增生Ⅰ～Ⅲ级和喉鳞癌进行了核面积、核仁面积、核DNA及核仁RNA相对含量的测定。结果显示:DNA-RNA含量,细胞核面积、核仁面积变化与病变程度一致,RNA参数在不典型增生Ⅱ、Ⅲ级阶段有明显增加,与DNA参数结合分析,可作为喉癌癌变阶段的监测指标。

The relative content of DNA-RNA and the nuclear area/nucleolar area ratio of the hyperplastic tissue,glottic polypus,dysplastic tissue of grade I to I laryngeal squamous cell carcinoma were measured in 33 cases with image analysis technique.It was found that the changes of DNA-RNA content and the ratio of nuclear/nucleolar area were parallel to those of the severity of the carcinoma,and RNA was markedly increased in grade 11 and I dysplastic tissue.On the basis of the current grading of laryngeal pathology,the combined determination of DNA-RNA can provide more sensitive indicators to the stages of malignant degeneration of the laryngeal squamous cell carcinoma.