摘要
从牛鼻软骨中获取HA-CLP复合物,4mol/L盐酸胍解离和亲和层析法纯化CLP。然后用SnCl2活化CLP后进行99Tcm离子螯合标记,获得了肺肿瘤显像探针99Tcm-CLP。结果显示:99Tcm-CLP的标记率和放射化学纯度均达到90％以上,体外与HA的亲和力KA为1.12×109L/mol,将99Tcm-CLP注入肺腺癌裸鼠体内发现在瘤区与HA有较好的浓集。表明99Tcm-CLP标记探针可用于肺肿瘤的显像研究。
The process of preparing an imaging micro-probe 99Tcm-CLP from bovine nose cartilage is described in detail. Both labeled rate and radiochemical purity of 99Tcm-CLP are greater than 90%, and KA is 1.12 × 109L/mol in vitro. After the Balb/c nu/nu mice with lung cancer were intravenously injected by the 99Tcm-CLP, the radioactivity was found to be well concentrated at the lung-cancer region, which suggests that the 99Tcm-CLP micro-probe can be used in imaging study of desmohemoblast on lung carcinoma.
出处
《核技术》
CAS
CSCD
北大核心
2004年第8期608-611,共4页
Nuclear Techniques
基金
广东省医药卫生科研基金项目(A2001482)资助
