冲击波对豚鼠螺旋神经节细胞P物质免疫反应的影响

Efiects of blast wave on SP-IR in spiral ganglion cells

作者采用免疫组织化学及图象分析技术发现,经压力峰值178.5 dB(SPL)的冲击波一次暴露后第1日,豚鼠螺旋神经节(SG)细胞P物质免疫反应(SP-IR)阳性产物的光密度较对照组明显增高(P<0.01),暴露后第3日,光密度较对照组为低(P<0.05),第7日恢复正常.暴露后各圈之间SP-IR产物的光密度无明显差异(P>0.05).免疫透射电镜发现爆震后第1日,豚鼠SGI型细胞胞浆内SP-IR产物明显增多,并可见较多的直径50～70nm的清亮小囊泡,有的囊泡包绕线粒体形成环形结构.爆震后不同时间组豚鼠的ABR阈移与SP-IR产物光密度的变化率成正相关(r=0.7251,P<0.05).结果提示冲击波所致听力损伤与SG细胞内SP的变化有关.

Fifteen guinea pigs were exposed to the blast wave of one shot of a 152 mm cannon with a peak pressure value of 178.5 dB (SPL). It was shown by an immunohistochemical method and an image analyzer that on the first day of the exposure, the optical density of the SP IR products was higher in the SG cells than that in the controls, and that it was lower on the third day (P<0.01 ). The optical density of the SP IR products become nor mal compared with that of the controls on the 7th day. No obvious differences could be found among the apical, middle, upper base and base. On the 1st day after the exposure, the SP IR products were found obviously increased in cytoplasm by immunoelectron microscopy. A lot of stained small clear vesicles (50~70 nm) existed in cytoplasm. Some of them formed a round structure around the mitochondrion. The ABR threshold of guinea pigs in different time groups was related to the changes of SP IR in SG cells (r=0.725 1, P<0.05). This suggests that the auditory damage induced by blast waves was correlated obviously to the changes of SP-IR in SG cells.