原发性肝细胞癌HBx基因缺失突变及意义

Deleted mutation and significance of hepatitis B virus X gene in primary hepatocellular carcinoma

作者应用聚合酶链反应技术分析了原发性肝细胞癌45例及其癌旁肝组织中HBc及HBx基因的存在形式.结果84.4％的癌及癌旁组织出现HBc扩增带,其中野生型分别占73.7%和81.6％,突变型分别占26.3％和18.4%.当同时扩增HBc加HBx基因时,癌及癌旁组织阳性率分别为71.1%和68.9%,其中野生型分别为25.0％和29.0％,突变型分别为75.0％和71.0％.表明HBx基因的缺失突变是一频发事件,其突变型和野生型的出现频率与单一扩增HBc基因比较,具有显著差异(P<0.01)、HBx基因的缺失突变,可能更有利于发挥反式激活作用,对于HBV的复制及原发性肝细胞癌的发生具有重要意义.

Hepatitis B virus core (HBc) and X (HBx) genes were detected in 45 cases of primary hepatocellular carcinoma (PHC) and their surrounding tissues by polymerase chain reaction amplification. Results indicated that 844% showed HBc gene fragment and among that wild type was 73.7% and 81.6%, mutant type was 263% and 184% in PHC and surrounding tissues, respectively. When HBc and HBx were amplified together, 71.1% and 68.9% were positive in PHC and surrounding tissues, wild type was only 25.0% and 29.0%, and mutant type was 75.0% and 71-0%, respectively. Results revealed that deleted mutation of HBx was frequent compared with HBc alone ( P<0.01). It suggested that deleted mutation of HBx gene may bring a cellular or virus gene close to the enhancer and act as a transactivator effectively, it is very important in hepatocarcinogenesis and replication of hepatitis B virus.