原发性肝细胞癌pYNZ22基因位点的多态性分析

Analysis of polymorphism of pYNZ22 gene in primary hepatocellular carcinoma

作者应用聚合酶链反应(PCR)和限制性酶片段长度多态性分析,检测了西北地区20例和启东地区(江苏)12例原发性肝细胞癌(PHC)的pYNZ22等位基因的杂合型丢失(LOH).结果西北地区20例PHC中14例为杂合型,2例出现LOH(14.3％);启东地区12例PHC中9例为杂合型,6例出现LOH(66.7％).二组病例的pYNZ22杂合型丢失频率有显著差异(P<0.05).西北地区为黄曲霉毒素低含量区,而启东地区为黄曲霉毒素高含量区.本结果表明,不同致病因素有关的PHC的基因变化存在差异,PHC的发生可能与多因素作用有关.

Twenty cases of primary hepatocellular carcinoma (PHC) from the Northwest China and 12 cases of PHC from Qidong, Jiangsu Province of China were examined for polymorphism of pYNZ22 gene by polymerase chain reaction and restriction fragment length polymorphism. The results indicated that 2 of 14 informative cases from the Northwest (14.3%) and 6 of 9 informative cases from Qidong showed loss of heterozygosity (66.7%), respectively. Frequencies of loss of heterozygosity in the two groups were different (P<0.05). The aflatoxin is low in the Northwest China, while high in Qidong, Jiangsu Province. This result suggested that the pathogenesis in the development of PHC is different, the multiple factors may be involved in hepatocarcinogenesis.