血凝抑制和巯基敏感试验快速检测甲型肝炎抗体

Rapid detection of hemagglutination inhibition antibodies against HAV by hemagglutination inhibition and 2ME-HAI test

作者应用血凝抑制试验(HAI)和巯基敏感试验(2ME-HAI)快速检测甲型肝炎(甲肝)血凝抑制抗体.以HAI与ELISA对比检测血清标本259份,总符合率为97%.甲肝感染者血清HAI滴度范围为1:200～51200.85％的甲肝急性期血清和96%甲肝恢复期血清HAI滴度≥1:6400,表明HAI滴度≥1:6400多为近期感染.2ME-HAI的结果为:甲肝急性期有98.3％的血清经2-ME处理后HAI滴度降低,其中降低倍数≥4占92%,而甲肝恢复期仅有17.4%的血清HAJ滴度降低,且仅降低2倍,提示2ME-HAI对甲肝的早期诊断有一定价值.

Hemagglutination inhibition (HAI ) and 2ME HAI were employed to detect hemagglutination inhibition antibodies against hepatitis A virus (HAV). Two hundred and fifty-nine sera were comparatively detected using HAI and ELISA and altogether 180 positive and 72 negative sera were found. The crude agreement, rate was 97%, HAI tilers in positive sera from individuals infected by HAV ranged from 1 :200 to 1 :51 200. 85% of acute-phase sera and 96% of convalesent-phase sera were with HAI tilers greater than 1 : 6 400. The findings suggested thai HAI titers higher than 1:6400 may indicate recent infection of HAV. HAI titers in 98.3% of acute-phase sera from patients infected with hepatitis A were reduced by 2-ME in 2ME HAI lest with 92% of them being reduced 4-fold while HAI tilers in 17.4% of convalescent-phase sera were reduced by 2-fold. These results showed that 2ME-HAI lest may be of certain value for early diagnosis of hepatitis A.