放射性微球技术测定维拉帕米和阿托品对兔内耳血流的作用

The effect of verapamil and atropine on blood flow of inner ear measured by the radioactive microsphere technique

有些内耳疾病如突发性耳聋、噪音性耳聋和Meniere病认为是由于内耳供血不足引起,临床常用钙拮抗剂和抗胆碱药治疗,以提高内耳血流量。作者采用^(51)Cr标记鸡红细胞为微球,用微球技术测定维拉帕米和阿托品对兔内耳血流的作用。结果:维拉帕米组平均内耳血流量为(0.40±0.27ml)/(g·min);阿托品组为(0.37±0.22ml)/(g·min);生理盐水组为(0.11±0.10ml)/(g·min).经统计学处理,维拉帕米和阿托品与生理盐水对照比较有显著差异(P<0.01),维拉帕米与阿托品之间无显著差异(P>0.05)。说明维拉帕米和阿托品能明显增进内耳血流量。

Several inner ear diseases such as sudden hearing loss, noise-induced hearing loss and Meniere's disease are thought to be due to the decrease of inner ear blood flow. Calcium antagonists and anticholinergic agents have been widely used to elevate inner ear blood flow. Our aim was to test verapamil and atropine to determine if they were effective on increasing blood flow in inner ear tissue of rabbits. The method employs the radioactive nuclide 51Cr labeled on RBC of chicken as biological microsphere technique. The result showed that the average blood flow of inner ear for verapamil was (0.40±0.37 ml)/(g min), for atropine was (0.37±0.22 ml)/(g min) and for normal saline was (0.11±0.10 ml )/(g min). It demonstrated that both drugs cause a significant increase in blood flow to inner ear (P<0.01).