可乐定对小鼠烫伤性休克的影响

Effect of clonidine on burn shock in mice

可乐定(Clo,1 mg/kg,ip)能显著抑制小鼠烫伤后血浆游离血红蛋白含量的增加,此作用可被育亨宾(5 mg/kg,ip)阻断而不受哌唑嗪(10 mg/kg,ip)的影响(P<0.01)。作者发现Clo能显著降低小鼠烫伤后红细胞的渗透脆性。50％溶血的盐水浓度:ClO组为 5.77±0.26 g/L。而生理盐水对照组(NS)为 6.57±0.21g/L(P<0.01)。预处理小鼠烫伤后,全血超氧化物歧化酶(SOD)的活性显著降低(P<0.01),并能抑制全血的超微弱发光强度60 s的积分值:Clo组为377.8±155.2;NS组为824.6±245.6,P<0.01;峰值:Clo组为 69.2±23.5 min;NS组 102.4±9.6 min,均P<0.01)。提示Clo可降低细胞内氧自由基的产生,从而减少脂质过氧化反应的程度,并降低小鼠烫伤后全血SOD的活性。

The effect of clonidine (1 mg/kg, ip) pretreatment on burn shock in mice was studied. The results showed that clonidine could significantly inhibit the increase of plasma free hemoglobin, this effect could be blocked by yohimbine (5 mg/kg, ip) while free of influence by prazosin (10 mg/kg, ip) (P<0. 01). We found clonidine could markedly decrease the osmotic fragility of RBC in postburn mice (Saline concentration causing 50% lysis of RBC:clonidine, 5. 77±0. 26 g/L vs control, 6. 57±0. 21 g/L, P<0. 01). Clonidine pretreatment could reduce the activity of superoxide dismutase notably in postburn mice (P<0. 01). It was also found that clonidine exhibited an inhibitory effect on the whole blood spontaneous chemiluminescence (Integral values of 60 s : clonidine, 377. 8±155. 2 vs control, 824. 6±245. 6, P<0. 01: Number of peaks/ min: clonidine, 69. 2±23. 5 min vs control, 102. 4±9. 6 min, P<0. 01). The results suggested that clonidine might decrease the production of intracellular oxygen free radicals, thus, reduce the extent of postburn lipoperoxidation. Clonidine pretreatment could reduce the activity of superoxide dismutase after burn.