聚合酶链反应检测乙型肝炎病毒DNA与ELISA检测e系统间的关系

The relationship between HBV DNA detected by PCR and e system detected by ELISA

作者应用聚合酶链反应(PCR)检测了89例慢性乙型肝炎e系统阳性患者血清乙型肝炎病毒(HBV)DNA,其中44例ELISA法HBeAg阳性者,PCR法HBV DNA检出率为88.10％;47例抗-HBe阳性者,PCR法HBV DNA检出率为65.96％。应用高特异、高灵敏的PCR法检测e系统阳性患者血清HBV DNA提示既往认为HBeAg阳性转为抗-HBe阳性做为病毒复制减弱或消失,病情缓解的指标是不确切的,而大部分抗-HBe阳性仍存在病毒复制及病情进展。

By using PCR. we examined HBV DNA in 89 chronic hepatitis B patients with positive e system. The positive rate of HBV DNA detected by PCR was 88. 1 % in 44 patients with positive HBeAg detected by means of ELISA while that in 47 patients with positive anti-HBe was 65. 96%. The above figures suggest that the traditional belief is doubtful that with the transition of positive HBeAg to positive anti-HBe, the duplication of virus would decrease or would even disappear. In fact, HBV duplication still exists and the disease is also progressive.