HCV结构蛋白重组杆状病毒表达载体的构建

Construction of a Recombinant Baculovirus Expression Vector of HCV Structural Protein

丙型肝炎病毒 ( HCV)结构蛋白的核心蛋白 ( C)和包膜蛋白 2 ( E2 )含有多个 T细胞表位和中和抗体表位 ,可诱导机体产生保护性免疫应答。该实验用 PCR扩增 HCV结构区基因 ,克隆到载体 p Fast Bac1中 ,构建了重组杆状病毒表达载体 p FB1 -CEE,为

Containing many T cell epi-positions and neutralizing antibody epi-positions, the core protein region and envelope protein 2 of Hepatitis C virus (HCV) structural protein may induce protective immunity responsion in body. In this experiment HCV structural gene was amplified by polymerase chain reaction(PCR), and was inserted into vector pFastBac1 to construct a recombinant baculovirus expression vector pFB1-CEE.