流行性出血热患者淋巴组织的病变及病毒抗原的分布

The pathology and viral antigen distribution in the lymphatic tissues of the patients died of epidemic hemorrhagic fever

作者用胶体金标记兔抗流行性出血热病毒多克隆抗体直接免疫金银法及抗病毒糖蛋白(G_2)单克隆抗体(MAb)LV48A和核蛋白(NP)MAb L133的多重PAP法观察了流行性出血热尸检的胸腺、脾脏及淋巴结。结果淋巴组织中胸腺上皮,脾及淋巴结的窦壁细胞,游离的单核巨噬细胞病毒抗原呈强阳性,胸腺及其它部位的淋巴细胞胞浆呈环状或点状阳性。淋巴组织明显萎缩,淋巴细胞减少,淋巴滤泡少见,未见生发中心,有的淋巴细胞核浓缩、崩解,但不见大片梗死样坏死。淋巴细胞浆细胞化现象很明显。淋巴组织同时有增生现象,表现为胸腺上皮巨细胞形成和个别淋巴细胞有核丝分裂。

The pathological changes and viral antigens in the thymuses, spleens and lymph nodes of the patients died of epidemic hemorrhagic fever (EHF) were studied by direct immunogold-silver staining method (D-IGSS) using gold labelled rabbit anti-EHFV polyclonal antibodies and repeated PAP immunocytochemical method using monoclonal antibodies (MAb) LV48A against viral glycoprotein I (G2) and MAb L133 a-gainst viral nucleocapsid protein (NP). The viral antigens were highly positive in the epithelium of thymus. fixed or wondering macrophages in spleens and lyrnph nodes, the viral antigens in lymphocytes were stained to be circular or spotted cytoplasma positive. The obvious atrophy of lymphatic tisues, decrease in number of lymphocytes, disapperence of germinal centers, karyopyknosis and karyolysis of lymphocytes could be seen besides there were proliferative changes of thymic epithelial cells with formation of giant cells. Mitosis and plasma cell transformation of lymphocytes could be also noticed. No massive infarct-like necrosis had been found in the lymphatic tissues.