低密度脂蛋白经不同方法氧化修饰后理化性质的改变

Variations of physical and chemical properties of LDL oxidatively modified with various methods

用Cu^(2+)诱导和小鼠腹腔巨噬细胞(MPM)共同孵育以及紫外线(UV)照射的方法都能够导致LDL的氧化修饰,表现在巴比妥酸反应物质(TBARS)、相对电泳迁移率(REM)、在420nm的荧光强度和经清道夫受体的降解速率等的增加。LDL的脂质过氧化修饰,除用作研究动脉粥样硬化的发生发展的机制外,还是一种研究脂质过氧化损伤和比较抗氧化剂的抗氧化效果及其作用机理的较为理想的模型。

After oxidative modification by Cu2+ or by incubation with mouse peritoneal macrophages (MPM) or by irradiation with ultraviolet light (UV), lipoperoxidation and molecular structure variations in low density lipoproteins(LDL) were observed,as the thiobarbituric acid reactive substances (TBARS) , relative electrophoresis mobility (REM), fluorescence emission spectra and degradation rate by scavenger receptors were all increased markedly. We think that oxidative modification of LDL is probably an ideal model for the study of lipoperoxidation, of the effect and the mechanism of antioxidant, as well as of the mechanism of atherogenesis.