LAK细胞体外杀伤直肠腺癌细胞的扫描电镜观察

SEM observation on in vitro LAK cells killing human rectal adenocarcinoma cells

应用扫描电镜观察体外LAK细胞与直肠腺癌细胞相互作用时的形态学变化。结果表明:LAK细胞与HR8348细胞均具有主动互相趋向运动,效靶细胞借其细胞突起及微绒毛相互接触。LAK细胞及肿瘤细胞的细胞突起及微绒毛由早期的平面接触逐渐发展为犬牙交错状结合。效靶细胞紧密结合后,靶细胞鼓泡,细胞膜出现环形穿孔。互相接触,结合的微绒毛及细胞突起在靶细胞的溶解过程中起到一个物理微桥的作用。LAK细胞分泌的杀伤物质通过微桥介导靶细胞的溶解。效靶细胞互相结合的微绒毛间形成关闭小室,它可能与维持局部杀伤物质浓度有关。

The morphological changes of lymphokine-activated killer(LAK) cells and human rectal ade-nocarcinoma (HR8348) cells were observed under scanning electron microscopy (SEM) during the interaction of the two kinds of cells in vitro. It was shown that the effector and target cells could move actively and chemotactically toward each other. LAK and HR8348 cells contacted with each other through cellular projections and microvilli. The LAK cells simply attached to the HR8348 cells at the early stage, followed by jigsaw-like binding of them. After conjunction, the target cells membrane appeared to blister and be fenestrated. The conjunction of microvilli and projections may act as physical micro-bridges in the lysis of the target cells, and killing factors may mediate the lysis by the micro-bridges, 'closed chamber' was formed between the conjunctive microvilli, which may play an important role in maintaining the local concentration of the killing substances.