连续流动法测定烟草水溶性糖的影响因素

Study on deviation source of determination of total sugars in tobacco products by continuous flow method

为提高RFA-300连续流动分析仪测定烟草及烟草制品中水溶性糖含量的准确性.依据行业标准YC/ T159—2002对各种影响因素进行了考察,发现标准样品的种类、纯度及反应时间是主要影响因素,并根据试验结果和测试原理的要求对仪器管路进行了适当的加长。确定了以葡萄糖为标样.采用校正标样和加长仪器管路的连续流动法测定烟草制品中的水溶性糖。结果表明,改进后方法的回收率为98.8%-107.9%,变异系数为1.63%. 测定结果与芒森·沃克法的基本一致。

The investigation was made on the relative factors which affect the accuracy of determination of water total soluble sugars in tobacco by continuous flow analysis. Meanwhile. the continuous flow method was improved based on the principle of the industrial standard YC/T159 -2002. The experimental results showed that the higher recovery and better repeatability were obtained comparing with former method