2,6-二叔丁基酚降解菌的降解特性研究

The Degradation Characteristics of Degrading Bacterium of 2,6-Di-tert- butylphenol

从腈纶废水处理构筑物的生物膜中分离、筛选得到 1株能降解 2 ,6 二叔丁基酚的菌株 ,经驯化 ,其对 2 ,6 二叔丁基酚的降解率提高了 2 6 % ,具有了较高降解能力 .经形态和生理生化鉴定 ,该菌株属于产碱菌属 (Alcaligenessp .) .通过摇瓶试验考察了生长条件对菌株的生长和底物降解的影响 ,得出该菌株的最适生长条件为温度 37℃ ,初始 pH为 7 0 ,接种量为 0 1% .在该条件下 ,对初始底物浓度为 10 0mg/L的降解过程进行了考察 ,结果表明其 11d的降解率达 6 2 4 % ,而且降解过程符合Ecken felder动力学模型 ,半衰期为 9 38d .还对不同初始底物浓度对菌株降解性能的影响进行了研究 ,结果表明最佳初始底物浓度为 2 0 0mg/L ,当小于该值时 ,初始底物浓度的增加促进该菌株的生长和底物的降解 ,而当大于这个值时 ,则起抑制作用 .

A degrading bacterial strain F-3-4 for 2,6-Di-tert-butylphenol(2,6\|DTBP) was isolated from biofilm in acrylic fiber wastewater treatment structures. By acclimation, its capacity for degradation of 2,6-DTBP was enhanced by 26%. It was identified as Alcaligenes sp. according to morphological, physiological and biochemical characters. By tests in shaking flasks, the effects of the conditions of growth was studied, and it was determined that the optimum conditions of growth for the strain was 37℃, pH 7.0 and inoculum amount 0.1%. Under these conditions, the kinetics of degradation for 2,6-DTBP of initial concentration 100mg/L was studied, and the result indicated that the removal rate of 2,6-DTBP within 11 days was 62.4%, and the degradation process followed Eckenfelder kinetics. The half life of 2,6-DTBP was 9.38 days. The effect of initial concentration on degradation ability of the strain also was investigated. The results showed that the optimum initial concentration was 200mg/L. When the initial concentrations were below it, the growth of strain and removal of 2,6-DTBP increased with the increase of initial concentration, while when above the value, they were inhibited.