膝沟藻毒素Gonyautoxins的分离纯化

Isolation and purification of gonyautoxins from Alexandrium mimutum Halim

目的 从微小亚历山大藻 (AlexandriummimutumHalim)Amtk 2中分离纯化膝沟藻毒素gonyautoxins。方法用凝胶色谱、离子交换色谱等方法从人工培养的微小亚历山大藻Amtk 2酸酒精萃取物中分离纯化膝沟藻毒素。结果 从 10 0LAmtk 2培养液中获得 ( 6 74± 0 31)× 10 9个藻细胞 ,其酸酒精萃取物经凝胶色谱分离首次在国内得到膝沟藻毒素GTX 4 ,GTX 1,GTX 3和GTX 2混合物 ( 2 9 5 9± 0 2 8)mg。从此混合物中取出 4 0 6mg经两次离子交换色谱纯化得到纯GTX 4 ( 0 4 0± 0 0 0 2 )mg ,GTX 1( 5 95± 0 0 3)× 10 - 2 mg ,GTX 3( 6 92± 0 0 5 )× 10 - 4mg和GTX 2 ( 0 11±0 0 0 5 )mg。结论 人工培养的微小亚历山大藻Amtk 2酸酒精萃取物通过凝胶色谱和两次离子交换色谱分离能够得到纯的膝沟藻毒素GTX 4 ,GTX 1,GTX 3和GTX 2。

Aim To isolate and purify gonyautoxins from Alexandrium mimutum Halim Amtk2 strain. Methods The ethanol extracts of culture Alexandriun minutum Halim Amtk2 were isolated by means of gel filtration chromatography, the toxin fraction obtained was then purified by ion exchange chromatography. Results From 100 liter of cultivation liquid of Alexandrium mimutum Halim Amtk2 (6 74±0 31)×10 9 cells were obtained. The ethanol extracts of Alexandriun minutum Halim purified by gel filtration chromatography obtained gonyautoxins mixture 29 59 mg. 4 06 mg of the mixture was further purified by two steps of ion exchange chromatography, and obtained pure GTX 4 (0 40±0 002) mg, GTX 1 (5 95±0 03)×10 -2 mg, GTX 3 (6 92±0 05)×10 -4 mg and GTX 2 (0 11±0 005) mg. Conclusion Pure gonyautoxins can be obtained by means of gel filtration chromatography and ion exchange chromatography from ethanol extracts of cultured Alexandriun minutum Halim Amtk2 strain.