微机极谱法同步测定血中铜 锌 铁的方法学探讨

Simultaneous Assay of Copper,Zinc and Iron in Serum by Micro-computerized Polarography

探讨Cu2 + 、Zn2 + 和Fe2 + 在乙二胺—三乙醇胺体系中的极谱联合测定方法 ,同时测定了 75 8例健康受试者中铜、锌和铁离子浓度。Cu2 + 、Zn2 + 和Fe2 + 在 0～ 4 g/L范围内线性较好 ,其检测极线分别是 7.8× 10 -5、5 .9× 10 -5和 8.2× 10 -6g/L ;样本分析的SD(CV)分别是 0 0 5 (4 5 6 % ) ,0 0 5 (4 5 9% )和 0 0 6 (4 5 1% ) ;回收率 (均值 )分别是 95 31%～ 10 2 4 8% (98 2 3% ) ,96 71%～ 10 1 34% (99 2 8% )和 98 16 %～ 10 3 95 %(10 1 92 % ) ;测定峰电位分别是 - 0 5 8V ,- 0 96和 - 1 35V。该方法与原子吸收分光光度法测定结果之间无统计学差异。

To report simultaneous determination of copper, zinc and iron in serum by micro-computerized polarography with the system of ethylenediamine-triethanolamine[H 2NCH 2CH 2NH 2-N(CH 2CH 2OH) 3], and assay the concentrations of copper, zinc and iron in serum from 758 healthy subjects.For copper, zinc, iron,with same linearty of range 0～4 g/L, the detection limits were 7.8×10 -5 g/L、5.9×10 -5 g/L,and 8.2×10 -6 g/L, the SD(CV) of sample analysis is 0.05(4.56%),0.05(4.59%), and 0.06(4.51%),the recoveries (mean)95.31%～102.48%(98.23%),96.71%～101.34%(99.28%), and 98.16%～103.95%(101.92%),the peak voltages were -0.58V,-0.96, and -1.35V,respectively. The difference between the results for the same sample by this method and the atomic absorption spectroscopic method was no statistical significance.