蛋白多糖在实验性动脉粥样硬化病变中的分布、超微形态定性和定量分析

Proteoglycan Distribution In Experimental Atherosclerotic Lesions、An Ultrastructural Quantitative & Qualitative Study

用钌红染色透射电镜观察的方法,在超微结构水平上对正常和不同时间高脂饲食免主动脉内膜蛋白多糖作定性和定量分析。结果表明:在正常和病变的主动脉内膜细胞间质均能清楚地看到两型钌红颗粒。大颗粒直径约20～100nm,有细丝状的突起相互联结成网,并和平滑肌细胞质膜、胶原及弹性纤维相联系。小颗粒直径小于20nm,表面光洁无突起。主要分布在内皮细胞、平滑肌细胞基底膜周围。含脂区颗粒较大,且与脂质相联系。定量发现在动脉粥样硬化病变早期,动脉内膜蛋白多糖含量增加,后期减少。本文探讨了蛋白多糖含量变化与脂质聚集的关系,认为动脉内膜蛋白多糖代谢紊乱是动脉粥样硬化发病的重要原因之一。

The content and distribution of proteoglycan in normal anddietary-induced experimental atherosclerotic aortic intimas of rab-bits were assayed quantitatively and qualitatively by electronmicroscope. The results showed: 2 types of ruthenium red-positivegranules were demonstrated in the intercellular matrix both in nor-mal and dietary-reduced atherosclerotic aortic intimas. A smallclass of ruthenium red-positive granule (less than 20 nm in dia-meter) was mainly present within the basement membranes beneaththe endothelium and surrounding the smooth muscle cell and smoothmuscle cell-derived foam cell; Large granules (20～100 nm indiameter) were dispersed in the intercellular matrix and wereinterconnected via their filamentous projections. They were intima-tely associated with the plasma membrane of intimal smooth musclecell or smooth muscle cell-derived foam cell and attached to colla-gen fibrils and elastic fibers. The articlc discusses the correlationbetween proteoglycan and lipid accumulation, and considers theproteoglycan metabolic disorder is one of the key agents of athero-sclerotic pathogenesis.