肾宁颗粒剂治疗大鼠C-BSA肾炎的药效学研究

Effects of shenning on rats with membranous glomerulonephritis

目的 观察肾宁颗粒剂对大鼠C BSA肾炎模型的治疗作用。方法 ♂Wistar大鼠 5 6只 ,随机分为正常组、模型组、肾宁颗粒剂高、中、低剂量组、肾复康组、泼尼松组 ,每组 8只。以阳离子化牛血清白蛋白 (C BSA)造成大鼠膜性肾炎模型 ,第 2wk末起每周测定 1次 2 4h尿蛋白 ,实验结束时测定血浆白蛋白、肾功能、前列腺素等 ,并做病理检查 ,观察各组治疗效果。结果 与模型组比较 ,肾宁颗粒可降低C BSA肾炎模型大鼠尿蛋白含量 ,以高剂量组起效最早 ,效果最显著。肾宁颗粒剂可升高模型大鼠血浆白蛋白含量 ,降低血浆肌酐和尿素氮含量 ,能降低C BSA肾炎大鼠血浆血栓素B2 (TXB2 ) /6 酮基 前列腺素F1α(6 Keto PGF1α)值 ,对肾功能有保护作用。结论 肾宁颗粒剂高、中剂量可明显降低膜性肾炎大鼠尿蛋白含量 ,高剂量可升高大鼠血浆白蛋白水平 ,在改善肾功能方面作用同肾复康作用相似 ,优于泼尼松。

AIM To investigate effects of shenning on nephrotic rats. METHODS Adult male wistar rats were randomly devided into normal control group, pathological pattern group, high-dosage shenning group,middle-dosage group,low-dosage group,shenfukang group and prednisone group. Rats with membranous glomerulonephritis were induced by injection of cation bovine serum albumin into tail vein. The rats were treated respectively for 32 days by gavage. Urinary protein was observed once a week after 2 weeks. After 7weeks ,plasma albumin,renal histology,parameters of renal function and prostaglandins were examined. In addition TXB 2/6-Keto-PGF 1α in serum were also examined. RESULTS The urinary protein,serum cretinine level,BUN and TXB 2/6-Keto-PGF 1α can be reduced in shenning groups compared with pathological pattern group. Plasma albumin was significantly increased in high-dosage shenning group. CONCLUSION High-dosage and middle-dosage shenning can significantly decrease urinary protein in rats with membranous glomerulonephritis. High-dosage shenning can increase the plasma albumin level in rats with membranous glomerulonephritis.