洛伐他汀、普罗布考对小鼠巨噬细胞源性泡沫细胞内胆固醇含量的影响

Effect of lovastatin and probucol on intracellular cholesterol content in foam cells derived from mouse peritoneal macrophages

目的 观察不同剂量下洛伐他汀和普罗布考对巨噬细胞源性泡沫细胞中胆固醇含量的影响 ,借此进一步探讨药物在促进胆固醇外流中的作用。方法 离体培养小鼠腹膜巨噬细胞 ,利用氧化的低密度脂蛋白蓄积胆固醇形成泡沫细胞 ,给予不同剂量的洛伐他汀与普罗布考后 ,利用高效液相方法测定细胞内胆固醇的含量。结果 模型组胆固醇酯(CE)的含量和胆固醇酯 /总胆固醇 (CE/TC)比值均较正常组和血清对照组升高 ,且CE/TC >5 0 % ,提示泡沫细胞模型建立成功。洛伐他汀可明显减少泡沫细胞内TC、CE含量 ,降低CE/TC比值 ,且有一定剂量依赖性。普罗布考可明显减少泡沫细胞内TC、CE含量 ,但无剂量依赖性 ,对CE/TC比值亦无明显影响。结论 洛伐他汀可促进泡沫细胞内胆固醇外流、降低细胞内胆固醇含量 ,减轻泡沫细胞化。普罗布考可降低细胞内胆固醇含量但对泡沫细胞化的影响不明显。

AIM To examine the effects of lovastatin, probucol on the intracellular cholesterol content and cholesterol efflux. METHODS With the model of foam cells through depositing the oxidized low-density lipoprotein (OXLDL) into mouse peritoneal macrophages, intracellular cholesterol content was examined by high performance liquid chromatography after the administration of lovastatin and probucol at different concentration. RESULTS The CE/TC ratios of the model group were beyond 50% and extremely higher than those of the normal and negative groups, suggesting that the model of foam cells was established triumphantly. Lovastatin decreased intracellular TC, CE and CE/TC significantly and dose-dependently. Probucol also possessed such effects on intracellular TC, CE, but no dose-dependently, and no effect on the ratio of CE/TC. CONCLUSION The results showed lovastatin might be conducive to plagues stability by increasing cholesterol efflux from the foam cells and decreasing the intracellular cholesterol content. Probucol did not have this effect although it decreased intracellular TC and CE content.