祛瘀化痰汤对肝纤维化大鼠肝组织α-平滑肌肌动蛋白表达、分布的影响的实验研究

A Study of Effects of Qu Yu Hua Tan Decoction on Distribution And Expression of Alpha-smooth Muscle Actin (α-SMA) in Liver Tissue of Fibrosis Rats

目的 :观察、研究祛瘀化痰汤对肝纤维化大鼠肝组织α—平滑肌肌动蛋白 (α -SMA)表达、分布的影响 ,以进一步探讨其防治大鼠肝纤维化的作用机制。方法 :实验动物分组为 :正常对照组 ;中药预防组 ;模型对照组 ;中药治疗组 ;模型自然恢复组。采用四氯化碳 (CCl4)诱导的大鼠肝纤维化模型 ,以免疫组织化学染色法观察各组大鼠肝组织α -SMA表达、分布情况。结果 :中药祛瘀化痰汤能显著减少中药预防组及中药治疗组大鼠肝组织α -SMA表达、分布 ,中药预防组及中药治疗组大鼠肝组织α -SMA表达、分布分别较模型对照组及模型自然恢复组明显减少 ,染色明显减淡 ,中药预防组与模型对照组、中药治疗组与模型自然恢复组之间比较 ,肝组织α -SMA表达、分布半定量分析及评分均存在显著性差异。结论 :祛瘀化痰汤能够通过抑制大鼠肝星状细胞的活化增殖 ,进而抑制胶原纤维合成而达到较显著的抗大鼠肝纤维化作用。

Objective: To observe the effects of qu yu hua tan decoction on distribution and expression of alpha-smooth muscle actin(α-SMA) in liver tissue of fibrosis rats by using immunohistochemical technique and to made semi-quantitative analysis and study of its possible mechanisms. Methods: The hepatic fibrosis rat's models were induced by subcutaneous injection of 40% CCl 4 oily solution. 60 Wistar rats were randomly divided into five groups: the normal control group, Chinese medicine preventive group, model group, Chinese medicine test group, model spontaneous reversion group. It was detected that the distribution and expression of alpha-smooth muscle actin(α-SMA) in liver tissue of every group rats by using immunohistochemical technique and its semi-quantitative analysis and scores were assessed. Results: Immunohistochemical stain showed the distribution and expression ofα-SMA in liver tissue was obviously less and the stains were markedly lighter in the chinese medicine preventive and the test group than that in the model and model spontaneous reversion group. There were all significant difference between the chinese medicine preventive and model groups, the chinese medicine test and model spontaneous reversion groups respectively in immunohistochemical semi-quantitative analysis and scores of α-SMA in liver tissue.Conclusion: The qu yu hua tan decoction has satisfactory effects on preventing and treating experimental liver fibrosis. The mechanisms of these effects could associate with its function of inhibiting the activation and proliferation of hepatic stellate cells and collagen synthesis.