^(125)I-脱氧尿嘧啶核苷对胰腺癌细胞Bax-Pc的杀伤作用

Killing effect of ^(125)I-UdR on human pancreatic cancer cell line Bax-Pc

目的 探讨1 2 5I 脱氧尿嘧啶核苷 (UdR)在胰腺癌细胞Bax Pc的特异性摄取及其杀伤细胞的能力 ,分析其作用条件和影响因素。方法 测量Bax Pc细胞和细胞核在含不同放射性浓度1 2 5I UdR的培养液中培养不同时间后的活度 ,评价其时间 效应和剂量 效应关系 ;用细胞克隆形成法评价1 2 5I UdR对Bax Pc细胞的杀伤作用。结果 ①Bax Pc细胞摄取1 2 5I UdR的量明显高于Na1 2 5I对照组 (P <0 0 1)。②细胞和细胞核中1 2 5I UdR的量随培养基中1 2 5I UdR放射性浓度增加而增加 (r =0 984～0 999)。③Bax Pc细胞和细胞核中1 2 5I UdR的量随培养时间增加而增加 (r=0 86 7～ 0 978)。④1 2 5I UdR组的存活分数明显低于Na1 2 5I对照组 (P <0 0 1) ,细胞存活分数有随培养基中放射性浓度和培养时间增加而降低的趋势。结论 1 2 5I UdR可被Bax Pc细胞特异性摄取并进入细胞核中 ,进而杀死细胞 ,其作用具有明显的时间 效应和剂量 效应关系。

Objective To evaluate the killing effect of 5-iodo-2′-deoxyuridine ( 125 I-UdR) in human pancreatic cancer cell line Bax-Pc in vitro and analyze the factors which affect the uptake of 125 I-UdR. Methods The amount of 125 I-UdR in cells and caryons was determined after incubated for 0.25～84 h in RPMI1640 culturing medium containing different concentrations of 125 I-UdR. The killing effects of 125 I-UdR on Bax-Pc were estimated through colony-forming assay. Results ①The uptake of 125 I-UdR by Bax-Pc cells was higher than that of Na 125 I control (P<0.01). ②The amount of 125 I-UdR in cells and caryons increased with the increasing concentration of 125 I-UdR in medium(r=0.984～0.999). ③The amount of 125 I-UdR in cells and caryons increased with increasing incubation time (r=0.867～0.978). ④The surviving fraction of 125 I-UdR groups were significantly lower than that of Na 125 I groups(P<0.01). Conclusions 125 I-UdR can specifically incorporated into DNA of Bax-Pc cells and kills the cells. The uptake of 125 I-UdR is dose and time dependent.