急性过量饮酒对大鼠心脏功能的影响

The effects of acute alcohol consumption on cardiac function in rats

目的 研究急性过量酒精摄入后大鼠心脏的病理、功能变化的特点及可能的机制。方法 2 8只成年雄性SD大鼠随机分成饮酒组与对照组。饮酒组按 5 35 7ml/kg体重 (即 2 4g纯酒精 /kg体重 )胃管灌入 5 6 %酒精 ,对照组给予同等量生理盐水。两组均分别于灌胃后 4 5和 12 0min通过颈总动脉插管观察左心室内压力、血压、心率和心肌力学指标的变化 ,同时测定血中酒精含量。实验结束后光镜下观察大鼠心脏病理变化。结果 ①饮酒组急性酒精摄入后 ,光镜下可见大鼠心肌细胞肿胀明显 ,出现嗜酸性变性。②与对照组比较 ,饮酒组急性酒精摄入后 4 5min时出现显著性的左心室压力指标下降 ,血压下降 ,心率减慢 ,心肌力学指标下降 (P <0 0 5 )。结论 ①酒精可对心肌细胞造成直接的损害作用。②急性过量酒精摄入可明显损害心肌收缩和舒张功能。

Abstract Objective To study the pathological changes of the heart and the changes of myocardial function caused by acute alcohol consumption.Methods Twenty-eight adult healthy Sprague-Dawley male rats, were randomly divided into different groups randomly. The alcohol(5.357ml/kg weight, 56% white wine) was administered through gastric tube in alcohol groups and equal volume of 0.9% physiological saline was given the same way to the control animals. The cardiac catheter technique was used through common carotid artery to measure the changes of the left ventricular pressure parameters, blood pressure, heart rate and myocardial mechanical parameters at 45min and 120min after treatment in both groups.lood samples were taken for alcohol concentration. Then the rats' hearts were removed and examined for pathology by HE staining. For statistical analysis the t-test and ANOVA were applied.Results 1. The myocardial cells looked swollen under the light microscopic examination at 45min after acute alcohol consumption, and the cytoplasm show acidophilic degeneration with HE staining. 2. Compared with the control groups, the parameters of the left ventricular pressure, blood pressure, heart rate and the myocardial mechanics in the alcohol groups were decreased most significantly at 45min after acute alcohol consumption. Conclusion 1. The alcohol directly damaged the myocardial cells. 2. Acute large amounts of alcohol consumption can damage the myocardial contractile and diastolic function.