L-选择素在体外循环中的表达及其拮抗剂的肺保护作用

Expression of L-selectin in rat models undergoing cardiopulmonary bypass and the pulmonary protective effect of its antagonist

目的 研究体外循环术后L 选择素的表达以及应用其拮抗剂进行肺灌注 ,探讨肺损伤发生的机制和阻断L 选择素表达所起的肺保护作用。方法 采用大鼠体外循环及术中肺灌注模型 ,观察手术前、后及应用拮抗剂后L 选择素的表达 ,组织超氧化物歧化酶 (SOD)、丙二醛 (MDA)、髓过氧化物酶(MPO)含量变化 ,氧指数 (PaO2 /FiO2 )及组织学改变。结果 体外循环术后L 选择素的表达增加 ,应用其拮抗剂进行肺灌注后其表达明显降低 ,肺损伤相应减轻。结论 L 选择素参与了体外循环中肺损伤的发生 ,阻断其表达可以减轻体外循环肺损伤 。

Objective: To study the expression of L selectin and its possible role in pulmonary injury in rat models undergoing cardiopulmonary bypass (CPB) and to investigate the protecive effect of fucoidin during the procedure. Methods: Models of CPB and pulmonary perfusion in rats were used to investigate the expression of L selectin pre and post operation. Its expression in antagonist group was also studied. Other indexes, including the concentration of SOD, MDA and MPO in rat lung tissues as well as PaO 2/FiO 2 and histological changes, were studied to illustrate the possible mechanism of L selectin in lung reperfusion injury. Results: The expression of L selectin increased after creating CPB but decreased after pulmonary infusion with fucoidin during the procedure, and lung injury relieved as well. Conclusion: L selectin might lead to lung injury during CPB, blocking its expression may relieve lung injury and enhance the recovery of lung function.