不同保存液对血管内皮细胞保护效果的实验研究

The experimental study of the protective effects of different storage solutions on rabbit endothelial cells

目的 研究不同保存液对血管内皮细胞超微结构和内皮层完整性的保护效果 ,以及对内皮素 1分泌水平的影响。方法 选取 16只健康成年家兔 ,取双侧股动脉 ,随机在异搏定 硝酸甘油复合溶液(VG)、含罂粟碱的肝素化全血 (PB)、罂粟碱溶液 (PS)和林格液 (Ringer)中保存 90min后做检测。 结果 PS组的绝大部分内皮细胞超微结构损伤明显 ,而PB组与VG组则大部分保护较好。内皮层覆盖比率VG组 [( 6 9± 10 ) % ]和PB组 [( 71± 9) % ]显著高于Ringer组 [( 5 5± 11) % ](P <0 0 1) ,而PS组 [( 4 8± 11) % ]与Ringer组比较差异无显著性 (P >0 0 5 )。内皮素 1分泌水平VG组 [( 4 1 4 13± 5 5 37) pg/ml]和PS组[( 6 6 6 2 8± 5 0 77) pg/ml]与Ringer组 [( 5 6 76 9± 5 6 91) pg/ml]比较差异均无显著性 (P >0 0 5 ) ;但PB组 [( 116 781± 2 2 387) pg/ml]显著高于Ringer组 (P <0 0 1)。 结论 PS对血管内皮细胞超微结构及内皮层完整性的保护效果欠佳 ,PB和VG对其保护效果较好 ,但PB显著增加内皮素

Objective: To investigate the protective effects of different storage solutions on the ultrastructure and integrity of rabbit endothelial cells, and their effects on endothelin 1 secretion. Methods: Femoral artery segments were harvested from 16 adult rabbits, which were then stored separately in four different storage solutions. The solutions was: (1) verapamil nitroglycerin solution (VG); (2) heparinized papaverine autologous blood (PB); (3) papaverine solution (PS); (4) Ringer's solution (Ringer). The ultrastructure of the endothelial cells,the covering percentage of endothelium monolayer and the level of endothelin 1 secretion were evaluated. Results: The covering percentage of endothelium monolayer was significantly higher in VG [(69±10)%] and PB[(71±9)%] compared with that in Ringer [(55±11)%] (P<0.01). The covering percentage was similar in PS [(48±11)%] and in Ringer (P>0.05). The endothelial ultrastructure was more seriously damaged in PS than that in VG and in PB through TEM. The level of endothelin 1 secreting was similar significantly increased only in PB [(116.781±22.387) pg/ml] compared with that in Ringer (P<0 01). Conclusion: Of all the tested solutions, PS might cause more serious damage than heparinized PB and VG solution in terms of the ultrastructure and integrity of the endothelial cells. Meanwhile, PB solution might exert an effect on the endothelin 1 secretion of the cultured rabbit endothelial cells. PB and VG got better preservation to endothelial ultrastructure, while PB kept higher level o endothelial secreting.