前列腺组织性激素结合球蛋白的研究

Studies on Sex Hormone-binding Globulin in the Prostate

目的 :旨在研究前列腺组织性激素结合球蛋白 (SHBG)的存在、含量 ,探讨SHBG的生理作用及与前列腺增生的关系。 方法 :应用12 5I标记单抗的双位点夹心免疫放射量度分析 (IRMA)方法 ,检测 2 2例正常中青年前列腺组织、11例老年对照组织和 32例良性前列腺增生 (BPH)组织的胞质和胞核提取液中SHBG的含量。组间差异采用t检验。 结果 :前列腺组织胞质中含有丰富的SHBG ,BPH组为 (5 .11± 1.37)fmol/g湿组织 ,略高于中青年组(4.98± 1.39)fmol/g湿组织和老年对照组 (5 .0 6± 1.2 7)fmol/g湿组织 ,但 3组间比较差异均无显著性 (P >0 .0 5 )。 结论 :前列腺组织中存在SHBG ;SHBG的存在有利于结合雄激素并使之在前列腺组织中得以积聚 ,与前列腺生理和前列腺组织增生有关。

Objective: To assay the existence of SHBG in prostatic tissue and analyze the effects of SHBG on the prostate and prostatic hyperplasia. Methods: Prostate tissue cytosolic and nuclear fractions were obtained from 32 cases of benign prostatic hyperplasia(BPH),11 cases of elderly control,and 22 cases of the normal young and middle-aged.SHBG in cytosol and nucleus was evaluated by two-site immunoradiometric assay based on the 125 I-labelled monoclonal antibody. Results: The study testified the existence of SHBG in the cytosol of prostatic tissue.SHBG in BPH tissue [( 5.11 ± 1.37 ) fmol/g wet tissue] was a little more than that in the young and middle-aged group [( 4.98 ± 1.39 ) fmol/g wet tissue] and the elderly group [( 5.06 ± 1.27 ) fmol/g wet tissue],but there was no significance among the three groups ( P > 0.05 ). Conclusion: SHBG in the prostate can bind and accumlate androgen,which is related to prostatic physiology and contributes to the etiology of BPH.