慢性前列腺炎患者前列腺液IL-1β和TNF-α的检测及意义

Clinical Evaluation of IL-1β and TNF-α in Prostatic Secretions for Chronic Prostatitis

目的 :了解IL 1β和TNF α在慢性前列腺炎患者前列腺按摩液 (EPS)中的变化及临床意义。 方法 :应用ELISA法对 34例慢性前列腺炎 [EPS中白细胞 (WBC)计数≥ 10 /HP为A组 16例 ,WBC <10 /HP为B组 18例 ]、10例无症状性前列腺炎、12例良性前列腺增生 (BPH)及 8例健康对照EPS中的IL 1β和TNF α进行检测。 结果 :IL 1β和TNF α在EPS中WBC≥ 10 /HP的慢性前列腺炎和无症状性前列腺炎两组检测值明显高于WBC <10 /HP的慢性前列腺炎、BPH和健康对照 3组 (P <0 .0 5 ,P <0 .0 2 )。IL 1β与TNF α有显著的数列等级相关性 (P <0 .0 0 3) ,而WBC计数和IL 1β、TNF α之间的数列等级相关性无显著性意义。 结论 :IL 1β与TNF α在伴有WBC计数增高的慢性前列腺炎患者EPS中明显增高 ,IL 1β与TNF α对传统以WBC计数为慢性前列腺炎进行分类的方法可以提供一个更准确的新分类方法。

Objective: To determine the clinical significance of interleukin-1β(IL-1β) and tumor necrosis factor-α(TNF-α) in expressed prostatic secretions(EPS) for chronic prostatitis. Methods: Prostatic secretions IL-1β and TNF-α were evaluated for 34 patients with chronic prostatitis,10 with asymptomatic inflammatory prostatitis,12 with benign prostatic hyperplasia(BPH) and 8 health controls by enzyme-linked immunosorbent assay(ELISA). Results: IL-1β and TNF-α levels in EPS in the patients of chronic prostatitis with WBC ≥10/HP and asymptomatic inflammatory prostatitis were obviously higher than those of chronic prostatitis with WBC<10/HP,BPH and health controls( P < 0.05 and P < 0.02 ). There was a correlation between IL-1β and TNF-α( P < 0.003 ) but none between WBC and IL-1β or TNF-α. Conclusion: Cytokines are frequently elevated in EPS in men of chronic prostatitis with high WBC and asymptomatic inflammatory prostatitis,which provides a novel means different from traditional methods based on WBC for the identification of men with chronic prostatitis.