胃癌组织血小板衍化内皮细胞生长因子、环氧化酶2的表达与血管生成和细胞凋亡的关系

Correlation of platelet-derived endothelial cell growth factor/ thymidine phosphorylase and Cox-2 expression to angiogenesis and apoptosis in gastric carcinoma

目的:探讨血小板衍化内皮细胞生长因子(platelet-derived endothelial cell growth factor,PD-ECGF)和环氧化酶2 (Cox-2)在胃癌组织中的表达及与血管生成和细胞凋亡的关系. 方法:应用免疫组化技术对67例胃癌组织进行PD-ECGF、Cox-2及肿瘤组织微血管密度(microvascular density, MVD)检测,流式细胞术检测胃癌细胞凋亡指数(apoptosis index,AI). 结果:PD-ECGF的表达与胃癌淋巴结转移(P<0.05)、分化程度(P<0.05)及组织分型(P<0.05)显著相关,Cox-2的表达与胃癌淋巴结转移(P<0.01)显著相关;二者与MVD (P<0.01)显著正相关,与AI(P<0.01)显著负相关,二者共同表达阳性者MVD显著高于共同表达阴性者(P<0.01), 二者共同表达阳性者AI显著低于共同表达阴性者(P<0.01). 结论:胃癌组织中PD-ECGF和Cox-2可促进血管生成, 抑制胃癌细胞凋亡,二者起协同作用,促进胃癌的增生与转移.

AIM: To investigate the relationship of the expression of platelet-derived endothelial cell growth factor/thymidine phosphorylase (PD-ECGF/TP) and Cox-2 to angiogenesis and apoptosis in gastric carcinoma. METHODS: The expression of PD-ECGF and Cox-2 and the microvascular density (MVD) level were examined by immunohistochemical staining, and apoptotic index (AI) was examined by flow cytometry in 67 cases of gastric carcinoma. RESULTS: There was a close correlation between PD-ECGF expression and several clinicopathological factors including lymph node metastasis (P<0.05), histology (P<0.05) and histological type (P <0.05). The expression of Cox-2 was closely related to lymph node metastasis (P<0.05); The expression of PD-ECGF or Cox-2 in gastric cancer tissues was positively correlated with MVD (P<0.01) and negatively correlated with AI (P <0.01). Combined analysis of PD-ECGF and Cox-2 showed that tumors with positive expression of both factors had a significantly lower AI and a significantly higher MVD than tumors with negative expression of both factors (P<0.01). CONCLUSION: Up-regulation of PD-ECGF and Cox-2 may facilitate tumor angiogenesis and inhibit apoptosis in gastric carcinoma, indicating cooperation between PD-ECGF and Cox-2 in gastric cancer, and therefore promote tumor growth and metastasis.