复方肝癌宁抗乙型肝炎病毒的体外实验研究

Anti-HBV effect of compound Ganaining in vitro

目的:探讨复方肝癌宁体外抗乙肝病毒(HBV)作用. 方法:通过复方肝癌宁作用于体外培养的2.2.15细胞,观察复方肝癌宁对2.2.15细胞分泌HBsAg、HBeAg的影响及药物的细胞毒性作用. 结果:复方肝癌宁作用于2.2.15细胞8 d后,对细胞的半数毒性浓度(TC印)为大于10 g/L,对HBsAg和HBeAg抑制的半数有效浓度(ICso)分别为0.67 g/L的0.13 g/L,复方肝癌宁对HBsAg和HBeAg的治疗指数(TU)分别为14.93和76.92. 结论:复方肝癌宁在体外细胞培养中对HBsAg和HBeAg的分泌有较好的抑制作用,有显著的抗HBV作用.

AIM: To study the anti-HBV effect of compound Ganaining in vitro. METHODS: The 2.2.15 cell line was cultured in vitro to observe the inhibitory effect of compound Ganaining on secretion of HBsAg and HBeAg, and its toxicity on 2.2.15 cell line was also studied. RESULTS: After the addition of compound Ganaining in 2.2.15 cell line for eight days, it showed that median toxic concentration (TC50) of compound Ganaining was more than 10 g/L. IC50 of compound Ganaining on HBsAg and HBeAg was 0.67g/L, and 0.13g/L, respectively; its treatment index (TI) value on HBsAg and HBeAg was 93.46 and 2.17, respectively. CONCLUSION: Compound Ganaining has a strong inhibitory action on secretion of HBsAg and HBeAg, and shows good effect of anti-HBV in 2.2.15 cell line.