摘要
目的:建立幽门螺杆菌(Hpylon)小鼠感染模型. 方法:以C57BL/6小鼠为实验动物,经口感染接种H pylori 悉尼株(SS1),置层流柜中饲养.用HE染色、石碳酸-碱性品红染色、免疫组织化学染色、尿素酶实验、细菌培养等方法检测接种小鼠,并用PCR、基因测序方法对胃组织细菌、胃组织分离培养细菌DNA进行分析. 结果:小鼠胃组织分离培养的细菌与接种细菌形态及特性相同.胃窦隐窝可观察到细菌定植,胃黏膜下层可见炎症细胞浸润.胃组织DNA中成功扩增出H pylori 16SrRNA及cagA基因的目的片段,基因测序结果显示与接种菌株序列完全一致. 结论:H pylori SS1经口接种C57BL/6小鼠2 mo后可成功在胃内定植并导致成慢性胃炎.
AIM: To establish a mouse model of H pylori infection. METHODS: C57BL/6 strain mouse, used as an experiment animal, was inoculated orally H pylori SS1 strain and fed in laminar flow cabinet. Inoculated mouse examined by HE stain, carbolic acid- basic fuchsin stain, immunohis-tochemical stain, urease test and bacteria cultivation. Both bacteria DNA from gastric tissue and H pylori isolated from stomach were analyzed by PCR and gene sequencing. RESULTS: The bacteria isolated from stomach had the identical shape and character compared with inoculated bacteria. Bacteria colonizing in antrun crypt and infiltration of inflammatory cells in gastric submucosa were observed. H pylori 16SrRNA and cagA gene were amplificated successfully from gastric tissue DNA, gene sequencing results showed the complete concordance. CONCLUSION: After inoculated into C57BL/6 mouse 2 months, H pylori SS1 can colonize successfully the mouse stomach and cause chronic gastritis.
出处
《世界华人消化杂志》
CAS
2004年第6期1313-1316,共4页
World Chinese Journal of Digestology
基金
国家自然科学基金资助项目
No.30271171国家教育部骨干教师基金资助项目
No.教技司2000-65湖南省自然科学基金资助项目
No.01jjy2114湖南省卫生厅科研基金资助项目
No.00022~~
