大鼠非酒精性脂肪肝中L-FABP的动态表达

Expression of liver fatty acid binding protein in rat nonalcoholic fatty liver

目的:研究L-FABP(liver fatty acid binding protein)在大鼠非酒精性脂肪肝形成中的作用. 方法:建立高脂饮食脂肪肝模型,用半定量逆转录聚合酶链反应(RT-PCR)与聚丙烯凝胶蛋白电泳(Westem Blot)方法测定脂肪肝肝组织中L-FABP表达变化. 结果:高脂饮食脂肪肝大鼠肝脏中L-FABP于2 wk时其mRNA及蛋白表达增强,于12 wk时表达最为明显,与正常组比较相差显著(1.42±0.034 vs 0.90±0.04;13 372.00±23.86 vs 6857.33±32.9 6637;P<0.05). 结论:高脂饮食引起L-FABP表达增强,最初是一种适应性反应,随着L-FABP表达进一步增强,导致脂肪酸代谢失衡,引起脂肪肝的发生.

AIM: To study the effect of liver fatty acid binding protein (L-FABP) in a model of rat nonalcoholic fatty liver. METHODS: Expression of L-FABP gene was examined in fatty liver rats by reverse transcription and polymerase chain reaction amplification and Western blot. RESULTS: In high-fat diet group, mRNA and protein expression of L-FABP increased at 2 wk, and elevated remarkably at 12 wk (1.42±0.034 vs 0.90±0.04; 13 372.00±23.86 vs 6857.33±32.96 637;P<0.05). CONCLUSION: Enhanced expressin of L-FABP at early staage is adapative reaction. With the advanced expression, disturbance of fatty acid metabolism may lead to nonalcoholic fatty liver.