摘要
火麻种子粉碎后经乙醇脱脂后用冷水抽提,经胃蛋白酶酶解后,透析和冷冻干燥得粗多糖H。利用离子交换柱DEAEsephadexA50(Cl-),琼脂糖凝胶SepharoseCL-2B和葡聚糖凝胶SephadexG-100对粗多糖H进行分离纯化,得到两种精制多糖HS1和HS2,并用凝胶过滤法测定了两种多糖的分子量,分别为42795和15396。多糖HS1经过气相色谱分析,结果表明其单糖组成主要为D-阿拉伯糖、D-木糖、D-甘露糖、D-半乳糖和D-葡萄糖组成,其摩尔比为0.12∶0.09∶0.15∶0.11∶0.12。
Hemp seed was defat by alcohol, water extracting, pepsin-enzymolysis, dialysing, freezing and drying. At last, the water soluble polysaccharides H was obtained. The crude polysaccharides H was fractionated by gel filtration chrom atograph on DEAE-sephadex A-50(Cl-) and Sepharose CL-2B and Sephadex G-100, two kinds of polysaccharides HS1 and HS2 were found. Furthmore, molecular weight was 42795 and 15396 which was determined by gel filtration chrom atograph method determined by gas chromatography the main ingredients of monosaccharide are D-Arabinose, D-Xylose, D-Mannose, D-Galactose and D-Glucose and the mole rate of them are 0.12∶0.09∶0.15∶0.11∶0.12.
出处
《食品科技》
CAS
北大核心
2004年第6期97-100,共4页
Food Science and Technology
基金
广西科学青年基金项目(0229002)。
关键词
火麻多糖
凝胶柱层析
分子量
单糖
polysaccharides of hemp
sephadex column chromatograph
molecular weight
monosaccharide
