解磷细菌突变株PS-01P菌株的选育及发酵条件的研究

Breeding of P-decomposing Strain and Exploration for Its Fermentation Condition

以PS 01P菌株为出发菌株,经过紫外线诱变,选育出一株解磷能力明显提高的菌株PS28P,其解磷量达257 24mg·L-1,比出发菌株提高了281 95%。通过对PS28P碳源、氮源、装液量和发酵温度等发酵条件的研究,确定了最佳发酵条件,即玉米粉0 5%,豆粕粉3 5%,鱼粉0 3%,装液量为50mL(250mL三角瓶),35℃培养40h为宜。菌量达到2 35×109mL-1。

PS28P which could produce 257.24 mg·L^(-1) P in shaking Flask was obtained from PS-01P through ultraviolet irradiation,and its phosphate decomposing capacity was 281.95% higher than that of starting strain.The factors including carbon sources ,nitrogen sources,culture temperature were studied and the results indicated that the optimum condition for fermentation is 0.5% maize powder, 3.5% bean powder, 0.3% fish meal and 50/250 mL medium volume at 35℃ for 40 h.Under this condition,bacteria number reachs 2.35×10~9 mL^(-1).