灰树花海藻糖合成酶基因的克隆及其在大肠杆菌中的表达

Cloning and Expression of Grifola frondosa Trehalose Synthase Gene in Escherichia coli

海藻糖主要作用是作为生物体的结构组分、以及保护生物膜和保护蛋白质。在灰树花中 ,海藻糖在干重中所占比例最高可达到 1 5 %～ 1 7% ,说明灰树花合成海藻糖的能力很强。将灰树花海藻糖合成酶基因克隆 ,并在大肠杆菌表达系统里表达。表达量为 1 90mg L。通过活性测定 ,证明在大肠杆菌中表达的海藻糖合成酶具有酶活性 ,结合基因工程和酶工程方法 。

Trehalose is a structural components of living organisms, and serves the function of protecting biological membranes and proteins. The dry weight of trehalose in Grifola frondosa can reach up to 15%～17%, indicating a strong ability to synthesize trehalose by trehalose synthase in the organism. Grifola frondosa trehalose synthase gene were cloned and expressed in Escherichia coli , trehalose synthase expression level reaching 190mg/L. The activity assay indicates that the enzyme expressed in bacteria possesses biological activity. This study has provided a new way for the enzymatic synthesis of trehalose.