美国黄松离体胚培养条件下不定芽的形成与根产生的研究

Adventitious Bud and Root Formation of Pinus ponderosa Cultured in vitro

以美国黄松成熟胚为外植体在MS、GD、SH和N6 培养基上诱导不定芽 ,试验结果表明 ,基本培养基的种类对外植体不定芽的诱导起主要作用 ,GD最好 ,SH次之 ,MS和N6 最差。GD +0 5mg·L- 1 6 BA ,对外植体不定芽的诱导率达 5 5 % ,平均增殖率为 6 ,最大增殖率达 10 ;NAA不利于外植体不定芽的诱导 ;培养基中加入适量的活性炭有利于不定芽的形成和生长。对不定芽在GD、SH和 1 2SH培养基上进行生根诱导 ,试验结果表明 ,基本培养基的种类对不定芽形成根起主要作用 ,GD、SH不能诱导不定芽生根 ,1 2SH可以使不定芽生根 ,其对不定芽的诱导率为 2 2 % ,1 2SH +NAA 0 5mg·L- 1 对不定芽生根的诱导率为 3 3% ;NAA对不定芽生根具有促进作用。在离体培养条件下 。

The mature embryo of Pinus ponderosa was used as explants to induce adventitious buds on GD、SH、MS and N 6 medium supplemented with 0.5～4.0 mg·L -1 6-BA and 0～1.0 mg·L -1 NAA.The results showed that the basic medium played a determinative role in initiating the adventitious buds. GD was best, SH better, and MS and N 6 worst. The optimal combination of the medium was GD with 0.5 mg·L -1 6-BA,on which 55% of explants was induced,average rate of propagation was six,the highest was ten. Addition of NAA would inhibit the formation of adventitious buds.A proper amount of active carbon promoted the formation and elongation of adventitious buds. The adventitious buds of P. ponderosa were used to induce adventitious roots on GD、SH、1/2 SH media supplemented with different concentration of NAA and IAA. The results showed that the basic medium played a determinative role in initiating the adventitious roots. Neither GD nor SH induced adventitious buds to form adventitious roots. Adventitious root was induced by 1/2 SH, on which 2.2% adventitious buds formed adventitious roots. The better combination of the medium was 1/2 SH with 0.5 mg·L -1 NAA, on which 3.3% adventitious buds formed adventitious roots. In this paper, the experimental result was reported that root was successfully induced from cultured embryo of P. ponderosa.