酶活化处理条件及其对松木纤维胶合性能的影响初探

Study of Wood-Activation with Laccase and the Effect on the Auto Adhesion of Pinus Fibers

通过测定漆酶处理纤维压制纤维板的内结合强度 ,观察到漆酶对云南思茅松木纤维的活化作用及漆酶处理对木纤维自身胶合的贡献。不同pH值条件下漆酶处理木纤维的内结合强度显著高于水处理纤维压制的板材强度。失活后的酶液与水等效 ,说明对实现木材自身胶合起作用的主要是漆酶本身。初步探索了pH值、酶剂量、底物浓度、活化处理时间等因素对胶合效果的影响。采用试验得到的较好活化条件压制的板材内结合强度可达1

The internal bonding strength (IB) of board made of laccase-reacted fibers (Pinus kesiya var. langbianensis),water_treated fibers,inacted laccase-treated fibers was compared.It was found that the internal bongding strength of fiberboards made from laccase_reacted fibers at pH 3.0,3.5,4.0,4.5, and 5.0 was markably higher than that from water treated ones and inactivated laccase_treated one.Therefore,the laccase activation of wood fibers and the contribution of laccase(EC 1.10.3.2)reaction to the auto adhesion of wood fibers were observed in the laboratory experiments.The main factors that effect on activation and adhesion,such as pH,dosage of laccase,the concentration of substrate and time for activation,were investigated through the test of IB.The fiberboard made under the optimal activating and hot_pressing conditions had desired internal bonding strength of higher than 1.0 MPa.