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二次回归正交设计在微型月季组织培养中的应用 被引量:23

Application of Quadratic Regressive Factorial Experiment to in vitro Culture of Miniature Rose
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摘要 Quadratic regressive factorial experiment was used to optimize concentrations of 6_BA and NAA added in MS midum for shoot multiplication of miniature rose (Rosa cv.Sonnen Kind).The most amount of shoots was achieved by combining 6-BA 1.93 mg·L -1, NAA 0.33 mg·L -1 into MS midum. The 6-BA and NAA concentrations were in a range of 1.68~2.75 mg·L -1 and 0.07~0.54 mg·L -1, respectively, and each 10 explants (stems with one auxiliary bud) cultured on the midum for 40 days, then 50 shoots occurred. The probability of the event was 95%. Quadratic regressive factorial experiment was used to optimize concentrations of 6_BA and NAA added in MS midum for shoot multiplication of miniature rose (Rosa cv.Sonnen Kind).The most amount of shoots was achieved by combining 6-BA 1.93 mg·L -1, NAA 0.33 mg·L -1 into MS midum. The 6-BA and NAA concentrations were in a range of 1.68~2.75 mg·L -1 and 0.07~0.54 mg·L -1, respectively, and each 10 explants (stems with one auxiliary bud) cultured on the midum for 40 days, then 50 shoots occurred. The probability of the event was 95%.
出处 《林业科学》 EI CAS CSCD 北大核心 2004年第4期189-192,共4页 Scientia Silvae Sinicae
基金 原林业部重点资助课题 ( 96 12 2 0 0 0 13 ) 国家重点推广项目 ( 2 0 0 2EC0 0 0 0 0 9 2 0 0 2EC0 0 0 0 10 ) 北京林业大学研究生基金资助项目
关键词 微型月季 组织培养 二次回归正交设计 激素 6-BA NAA 培养基 Quadratic regression factorial experiment, Miniature rose, in vitro culture, 6_BA, NAA
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  • 1朱鹿呜,园艺学报,1985年,4期,273页
  • 2杨文政,河南农学院学报,1984年,2期,8页
  • 3蔡军,植物生理学通讯,1984年,5期,37页
  • 4林证明,园艺学报,1982年,9卷,2期,67页

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