大剂量维生素C与小剂量甲基强的松龙联合治疗大鼠急性脊髓损伤初步研究

The effects of therapy with high-dose Vitamin C combined with low-dose methylprednisolone for acute spinal cord injury in rat

目的:研究大剂量维生素C(VitC)+小剂量甲基强的松龙(MP)对大鼠急性脊髓损伤(SCI)的疗效。方法:SD成年大鼠64只,采用Allen法(7g×4cm)在T11造成急性SCI动物模型。动物随机分为:MP治疗组(A组)﹑VitC+1/2MP治疗组(B组)﹑VitC治疗组(C组)﹑对照组(D组)。伤后48h行脊髓形态学检查(HE染色+Olsiwski氏小体染色)、测定脊髓含水量,伤后2周进行行为学检查(斜板实验+Gale评分)。结果:B组SCI后48h脊髓坏死及出血范围明显轻于D组,伤后2周行为学指标优于D组(P<0.05),与A﹑C组相似(P>0.05);伤后48h脊髓含水量明显低于A组﹑C组﹑D组,神经元残存Olsiwski氏小体数量明显高于A组﹑C组及D组(P<0.05)。结论:大鼠急性SCI后早期使用大剂量VitC+小剂量MP具有治疗作用,效果优于单独应用大剂量MP或VitC治疗。

Objective:To investigate the effects of therapy with high-dose Vitamin C(Vit C)combined with low-dose methylprednisolone(MP)in acute spinal cord injury(SCI).Method:Fourty-eight rats were divided into group A﹑B﹑C﹑D,and SCI was made by Allen's mode(7g×4cm)on spinal cord T11extradually.0.5h after SCI,group A﹑B﹑C﹑D received MP(30mg/kg,i.p),Vit C(200mg/kg,i.p)+MP(15mg/kg,i.p),Vit C(200mg/kg,i.p),and SCI re-spectively.Hemetoxylin staining+Olsiwski corpuscle staining and moisture of spinal cord48h after SCI were ob-served.Inclined plate+Gale scale were observed2-week after SCI.Result:48h after injury,in group B,there was less degree of hydrops,more Olsiwski corpuscle count in neure of injuried aeras than in other groups(P<0.05),and less scope of neuro-necrosis and bleeding in the injuried spinal cord areas in comparison with group D.2week after injury,in group B,there was better behavioral assessments(inclined plate+Gale scale)than in group D(P<0.05),while no difference was found among group A﹑B﹑C statistically(P>0.05).Conclusion:Early high-dose Vit C combined with low-dose MP is cabable of treating SCI,and is superior to high-dose MP or Vit C.