基质金属蛋白酶-2,9在下咽癌中的表达及其与淋巴结转移的相关性

Expression of matrix metalloproteinase-2,9 in hypopharyngeal carcinoma and its relationship with lymphatic metastasis

目的 :探讨基质金属蛋白酶 2 ,9(MMP 2 ,9)与下咽癌组织生物学行为及其与淋巴结转移的相关性。方法 :运用免疫组织化学二步法检测下咽癌组织中MMP 2 ,9抗体的表达。结果 :32例下咽癌组织中MMP 2表达阳性率为 5 3.12 % ,MMP 9为 5 9.38%。MMP 2 ,MMP 9在癌旁正常黏膜中与下咽癌组织中的表达差异均具有统计学意义 (均P <0 .0 1) ;发生淋巴结转移者与未发生转移者的染色差异有统计学意义 (P <0 .0 5 )。结论 :MMP 2 ,9可能与下咽癌的淋巴结转移有关。

Objective:To study the relationship between matrix metalloproteinase 2,9(MMP 2,9) and the biology behavior, lymphatic metastasis of hypopharyngeal carcinoma by investigating the expression of MMP 2,9.Method:Two step immunohistochemical method was used to examine the expression of monoclona antibody in hypopharyngeal carcinoma tissues.Result:It was found that the positive rate of MMP 2 in 32 hypopharyngeal carcinoma tissues was 53.12 %(17/32),and the positive rate of MMP 9 was 59.38 %(19/32). The difference of the expression in tumor tissue and normal mucosa beside the tumor was significant. The positive rate in those carcinoma with cervical lymph node metastasis was much higher than that in cases without cervical lymph node metastasis. And there was no significant difference found in the expression of MMP 2 and MMP 9.Conclusion:MMP 2 and MMP 9 may relate with the lymph node metastasis in hypopharyngeal carcinoma.