头颈部肿瘤DNA的定量分析

DNA quantitative analysis of head and neck tumor

目的 :探讨细胞定量学分析在诊断头颈部肿瘤中的意义。方法 :应用全自动细胞图像分析仪测定2 3例头颈部恶性肿瘤和 2 0例良性病变患者的细胞核DNA指数 (DI)、S期细胞分数 (SPF)、细胞增殖指数 (PI)和DNA大于 5c的核的比率 (5cEx)。结果 :细胞定量分析和常规细胞学检查的阳性率分别为 76 .92 %和 5 0 .0 0 % ,SPF、PI和 5cEx在恶性病变组分别为 14 .0 5 %、19.5 9%和 1.5 9% ,SPF和PI在肿瘤部位的细胞增生程度高 ,与瘤旁 1cm和手术切缘处存在明显不同。结论 :全自动细胞图像分析仪的细胞定量诊断阳性率较常规细胞学检查明显提高 ,异倍体率、SPF、PI和 5cEx指标在诊断头颈部肿瘤、估计肿瘤的生物学恶性程度和评估手术范围等临床工作中有一定参考价值。

Objective:This research discussed the diagnostic value of cellular quantitative analysis in head and neck tumor.Method:We analysis quantitative parameters of 23 malignant tumors and 20 benign diseases with automated DNA image cytometry,they are the DNA Index (DI),S phase Fraction (SPF),Proliferation Index (PI) and 5c Exceeding (5c Ex).Result:The masculine rate of cellular quantitative analysis and conventional method are 76.92 % and 50.00 %. The value of SPF,PI and 5c Ex are 14.05 %, 19.59 % and 1.59 % in malignant tumor,respectively. The parameters of SPF and PI showed distinct difference in the area of malignant tumor than the 1cm sideward area of tumor and the edge of tumor.Conclusion:Automatic DNA image cytometry has higher masculine rate of malignant tumor than conventional method in early detection of tumor. The cellular quantitative parameters, such as SPF,PI and 5c Ex, are able to guide clinical work, specially in diagnosing of head and neck tumor,estimating biological character of malignant tumor and evaluating the area of excision.