苯作业工人胸腺近期输出功能评价

Evaluation of recent thymic output function in benzene-exposed workers

目的 了解苯作业工人外周血单个核细胞 (peripheralbloodmononuclearcells ,PBMCs)中信号结合T细胞受体删除DNA环 (signaljointT cellreceptorexcisionDNAcircles,sjTRECs)的含量 ,推测苯作业工人幼稚T细胞含量和胸腺近期输出功能情况。方法 利用实时定量PCR和TaqMan方法检测 11例健康人和 6 2例苯作业工人每 10 0 0个PBMCs中sjTRECs的含量。结果 未经校正苯接触组sjTRECs含量 (中位数为 2 .5 6 )明显低于正常人 (中位数为 7.81) ,差异有显著性 (P <0 .0 1)。苯接触组年龄≤ 30岁者sjTRECs含量 (3.19 10 0 0PBMCs)明显高于年龄 >30岁者 (1.76 10 0 0PBMCs ,n =2 3) ,差异有显著性 (P <0 .0 5 )。结论 苯或苯化合物接触影响胸腺近期输出功能 ;sjTRECs含量与苯作业工人年龄有关。

Objective To analyze the content of signal joint T-cell receptor excision DNA circles signal joint T-cell receptor excision DNA circles(sjTRECs) within peripheral blood mononuclear cells (PBMCs),thereby to infer the level of naive T cells and the recent thymic output function in benzene-exposed workers. Methods Quantitative detection of sjTRECs in DNA of peripheral blood mononuclear cells from 11 normal individuals and 62 benzene-exposed workers were performed by real-time polymerase chain reaction(PCR) and TaqMan technique. Results The median value of sjTRECs copies/1 000 PBMCs was 7.81 in normal individuals whereas it was 2.56 copies/1 000 PBMCs in age-unadjusted benzene-exposed workers(P<0.01).And its levels were obviously different between two different age groups:that in 30-year-old group(1.76 copies/1 000 PBMCs,n=23) was less than ≤30-years-old group(3.19 copies/1 000 PBMCs,n=39,P<0.05). Conclusions Benzene exposure may influence the recent thymic output function,and the sjTRECs levels may relate with the age of benzene-exposed workers.