人参皂苷Rg1对脂多糖诱导的C6细胞株淀粉样前体蛋白和脑啡肽酶表达的影响

Effect of ginsenoside Rg1 on the amyloid protein precursor and neprilysin expression induced by lipopolysaccharide in C6 cell line

目的 :为寻找治疗阿尔茨海默病的有效药物 ,探讨人参皂苷Rg1对脂多糖 (LPS)诱导的C6细胞株淀粉样前体蛋白 (APP)和脑啡肽酶 (NEP)表达的影响。方法 :应用MTT比色法检测一定剂量LPS(10 0mg·L-1)和不同浓度人参皂苷Rg1(2 5、5、10、2 0 μmol·L-1)对C6细胞存活率的影响 ,应用RT -PCR观察细胞中APP、NEP表达的变化。结果 :LPS作用于C6细胞株 ,C6细胞存活率下降 ,细胞内APP的表达增加 ,NEP的表达降低。人参皂苷Rg1能提高LPS处理的C6细胞存活率 ,使细胞内APP的表达降低 ,NEP的表达升高。结论 :LPS造成细胞损伤和细胞内APP表达增加、NEP表达降低 ,人参皂苷Rg1对LPS造成的细胞毒性具有拮抗作用 ,可减弱LPS引起的细胞内APP表达增强 ,减轻LPS对细胞内NEP表达的抑制。

AIM: This study was designed to investigate the effect of ginsenoside Rg1 on the amyloid β-protein precursor (APP) and neprilysin (NEP)expression induced by lipopolysaccharide (LPS) in C6 cell line in order to discover effectual Alzheimer's disease (AD)-treated drugs. METHODS: MTT colorimetric analysis was used to measure the survival rate of C6 cultured with ginsenoside Rg1 at different concentrations (2 5, 5, 10 and 20 μmol·L -1) and LPS (100 mg·L -1). The expression of APP and NEP mRNA was measured by RT-PCR. RESULTS: LPS decreased the survival rate of C6, furthermore, the increase in APP expression and the decrease in NEP expression were observed. On the other hand, the above alteration induced by LPS was reversed by ginsenoside Rg1. CONCLUSION: This study demonstrates that LPS can cause cell damage, the increase in APP expression and the decrease in NEP expression. Ginsenoside Rg1 can exert a neuroprotective action, protect C6 cells against LPS-induced injury via inhibiting APP expression and increasing NEP expression.