脱水猪角膜基质为载体体外构建猫角膜内皮组织

Reconstructed corneal endothelial tissue on allo-stroma in vitro

目的 :以脱水猪角膜基质为载体体外构建猫角膜内皮组织 ,并观察其形态结构 ,以寻找体外构建角膜组织最佳载体材料及方法 ,最终目的是体外构建可用于移植的角膜内皮组织。方法 :以脱水处理的猪角膜基质片为载体 ,将猫角膜内皮细胞接种于去除猪角膜内皮细胞的后弹力膜上 ,在添加了表皮生长因子和层粘连蛋白的培养液中培养 7d ,分别观察倒置显微镜下、组织学切片及扫描电镜下内皮组织的形态结构。结果 :倒置显微镜下 ,组织培养的猫角膜内皮细胞形成单层内皮组织 ,排列较规律 ,细胞间连接紧密 ;组织学观察发现 ,培养的猫角膜内皮细胞形成完整的内皮组织 ,贴附于脱水基质的后弹力膜上 ,与正常的角膜内皮组织结构相似 ;扫描电镜下 ,组织培养的猫角膜内皮细胞间连接紧密 ,细胞大小不甚一致 ,胞核清晰。结论 :以脱水猪角膜基质为载体 ,体外成功构建出猫角膜内皮组织 ,其形态结构近似于正常角膜内皮组织。

AIM: In order to search carrier material and better tissue culture method, the morphology and structure of the cultured cat corneal endothelium was observed. METHODS: The cat corneal endothelial cells were seeded on the Descemet's membrane of the dehydrated swine corneal stroma, and then cultured in the medium with epidermal growth factor and laminin for 7 days. The morphology and structure of reconstructed tissue was tested by the inverted microscope and the scanning electron microscope. RESULTS: As shown by the morphological observations, the cultured cat corneal endothelial cells formed an integrated membrane, and attached to the Descemet's membrane, which was similar to the nature tissue. The cells connected tightly to each other, and some of them arranged in hexagon approximately. CONCLUSION: The cat corneal endothelial cells could be rebuilt on the carrier of the dehydrated swine corneal stroma successfully, which is similar to the nature tissue.