α_1胸腺肽对胸腺细胞发育和成熟的影响

Effects of thymosin α_1 on the development and maturation of thymocytes

目的 :探讨α1胸腺肽对胸腺细胞发育的影响。方法 :测定CD4 + CD8+ 胸腺细胞群的比例及 5 -FU和胸腺肽处理组在CD4 -CD8-胸腺细胞群上Hh信号的Smo分子 (增生反应的潜在阴性调节者 )的表达效果来估价胸腺肽对胸腺细胞成熟和发育的影响。结果 :发现α1胸腺肽在 30 μg/kg的低剂量下显示活性。流式细胞仪分析根据CD4 -CD8-胸腺细胞上Hh信号的Smo分子的表达确定α1胸腺肽在此剂量加快了胸腺细胞的成熟。结论 :α1胸腺肽能加快CD4 -CD8-胸腺细胞向CD4 + CD8+ 阶段的发展。

AIM: To investigate the effect of Thymosin α 1 on the development and matutation of thymocytes. METHODS: The proportion of CD4+CD8+ thymocytes and the expression of smoothened (Smo) of the hedgehog (Hh)-signaling in CD4-CD8-thymocytes were examined to observe the effect of thymosin α 1 on thymocyte development and matutation. RESULTS: Flowcytometric analysis showed that thymosin α 1 showed activity at a low dose of 30 μg/kg, and 30 μg/kg thymosin α 1 accelerated the replenishment and maturation of thymocytes according to the expression of Smo of the Hh-signaling in CD4-CD8-thymocytes, the potent negative regulator of proliferative responses. CONCLUSION: Thymosin α 1 can accelerate thymocyte development from CD4-CD8- to CD4+CD8+.