肝细胞癌患者血清VEGF水平与侵袭和转移的相关性研究

Study on expression of the serum vascular endothelial growth factor(VEGF) correlates with invasion and metastasis of hepatocellular carcinoma

目的 探讨肝细胞癌 (HCC)患者血清中血管内皮生长因子 (vascularendothelialfactor,VEGF)的表达水平与肝癌侵袭和转移的关系。方法 应用ELISA法对 80例HCC、6 8例肝脏良性疾病患者和 4 0例健康人的血清标本进行VEGF活性检测。结果 HCC组血清VEGF水平〔(42 9 6 1± 2 12 18)pg ml〕 ,与肝脏良性疾病组〔(15 3 4 7± 89 77)pg ml〕及健康人组〔(116 79± 5 6 39)pg ml〕比较 ,差异均有显著性 (P值均 <0 0 1) ;HCC转移组患者血清VEGF表达水平与未转移组相比 ,差异有显著性 (P <0 0 1) ;血清VEGF表达水平还与HCC合并门静脉瘤栓、肿瘤大小和TNM分期密切相关 ,VEGF含量随TNM分期升高而逐步升高。结论 VEGF在HCC浸润和转移过程中发挥重要作用 ,术前检测血清VEGF水平对预测HCC的侵袭和转移具有重要的临床意义。

Objective This study was to investigate the relationship between the expression of serum vascular endothelial growth factor(VEGF) and invasion and metastasis of the patients with hepatocellular cacinoma(HCC).Methods The level of serum VEGF was measured with the quantitative sandwich enzyme linked immunosorbent assay(ELISA kit) in 80 patients with hepatocellular cacinoma(HCC), 68 patients with benign liver lesions and 40 healthy control. Results Serum VEGF level in HCC〔(429.61±212.18)pg/ml〕 was significantly elevated as compared to those in patients with benign liver lesions〔(153.47±89.77)pg/ml〕 and those of the normal controls〔(116.79±56.39)pg/ml〕(P<0.01), though the mean VEGF level of benign liver lesions and normal controls were not significantly different. Serum VEGF level in patients with portal vein(PV) emboli, with metastasis or with large HCC lesions were significantly higher than those without PV-emboli, without metastasis or with small HCC lesions(P<0.01). Serum VEGF level increased with the TNM stage(P<0.01).Conclusion VEGF may play an important role in the invasion and metastasis of HCC. Serum VEGF assay may be used to preoperatively predict invasion and metastasis of HCC.