摘要
应用聚合酶链式反应技术穴PCR雪扩增了玉米淀粉分支酶的cDNA基因片段,并将其克隆到pMD18-Tvector载体上,对重组子进行PCR检测和限制性内切酶分析,并测定了DNA全序列。结果表明:克隆片段全长为1698bp。将该基因反向插入到pCAMBIA1301的CaMV35S启动子之后,构建了反义表达载体。
cDNA segment of corn starch branching enzymes gene was obtained by PCR techinque and cloned into pMD18-Tvector.The recombinant clone was detected by PCR technique and analysed by the restriction en-zyme.A full length of cDNA gene was sequenced.The results showed that the length of the clone sequence is1698bp.The gene was inserted into binary vector in reverse orientation and constructed antisense vector.
出处
《玉米科学》
CAS
CSCD
2004年第2期105-107,共3页
Journal of Maize Sciences
基金
"国家转基因植物中试与产业化基地建设"专项基金(J99-13-001)