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玉米淀粉分支酶基因的克隆和反义载体的构建 被引量：4

Cloning and Cons truction of Antisense Vector on Gene of Corn Starch Branching Enz ymes
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摘要 应用聚合酶链式反应技术穴PCR雪扩增了玉米淀粉分支酶的cDNA基因片段,并将其克隆到pMD18-Tvector载体上,对重组子进行PCR检测和限制性内切酶分析,并测定了DNA全序列。结果表明:克隆片段全长为1698bp。将该基因反向插入到pCAMBIA1301的CaMV35S启动子之后,构建了反义表达载体。 cDNA segment of corn starch branching enzymes gene was obtained by PCR techinque and cloned into pMD18-Tvector.The recombinant clone was detected by PCR technique and analysed by the restriction en-zyme.A full length of cDNA gene was sequenced.The results showed that the length of the clone sequence is1698bp.The gene was inserted into binary vector in reverse orientation and constructed antisense vector.
作者 柴晓杰 王丕武 张君 关淑艳
机构地区 吉林农业大学生物技术学院
出处 《玉米科学》 CAS CSCD 2004年第2期105-107,共3页 Journal of Maize Sciences
基金 "国家转基因植物中试与产业化基地建设"专项基金(J99-13-001)
关键词 玉米淀粉分支酶 CDNA 反义载体 聚合酶链式反应技术 PCR 克隆 Starch branching enzymes(SBE)gene PCR Clone cDNA Antisense vector
分类号 S513 [农业科学—作物学]
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参考文献7

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共引文献79

同被引文献51

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玉米科学
2004年 第2期

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