薄层扫描法测定尔胃康颗粒中人参皂苷R_(g1)的含量

Determination of ginsenoside Rg1 in Er-wei-kang Granules by TLC scanning

目的 建立测定尔胃康颗粒中人参皂苷Rg1含量的方法。方法 对照品溶液与供试品溶液分别点于同一硅胶G薄层板上,以氯仿-醋酸乙酯-甲醇-水(2:4:2;1)放置后的下层液为展开剂,展开,取出,晾干,喷以10％硫酸乙醇溶液,在105°C加热至斑点显色清晰,用同样大小的玻板密封,用薄层扫描仪在λs=530nm,λR:700nm下测定。结果人参皂苷Rg1在1.13-6.78μg范围内线性关系良好,r=0.9991,本方法的平均回收率为97.3％,RSD为1.69％。结论该方法灵敏、准确、专属性强、重现性好,可用于本制剂的含量测定及质量控制。

Objective: To establish a method for the determination of ginsenoside Rg1 in Er - wei - kang Granules. Methods: The sample was applied on a silica gel G plate and developed with the bottom of chloroformethyl acetate - methanol - water( 2:4:2:1) and was sprayed with sulfuric acid containing ethyl alcohol. The separarated spot was determined by scanning with CS - 930 at λs = 530nm, λR = 700nm. Result and Conclusion: Ginsenoside Rg1 showed a good liner relationship at a range of 1.13 - 6.78μg, r = 0.9991. The average recovery was 97. 3% ,and RSD was 1.69% .Conclusion:The results showed that this method is sensitive,specific,accurate and reproducible.